Biotechnology - MCQ Practice Questions
Biotechnology is one of the few subjects where exam questions track laboratory practice closely, so the techniques are worth knowing in order. Practice covers recombinant DNA technology, PCR and gel electrophoresis, cloning vectors, tissue culture, fermentation and bioprocess technology, and applications in medicine and agriculture. Technique questions explain why a step exists, not just what it produces.
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Which DNA amplification technique is used to amplify sequences adjacent to a known sequence boundary?
In droplet digital PCR (ddPCR), the sample is partitioned into thousands of micro-compartments. What is the primary advantage of this approach?
Which DNA technology would be most appropriate for detecting point mutations and SNPs in cancer genomics?
What is the primary challenge in developing universal primers for metagenomic studies of microbial communities?
Which modification to standard PCR allows amplification of extremely long DNA fragments (>3 kb)?
Which PCR-based technique would be most suitable for detecting mutations associated with antibiotic resistance in Mycobacterium tuberculosis in clinical samples?
In the amplification of GC-rich genomic regions, which strategy is most effective to reduce secondary structure formation?
A researcher is performing digital PCR (dPCR) for absolute quantification of mutant KRAS alleles in circulating tumor DNA (ctDNA). Compared to conventional qPCR, what is the primary advantage of dPCR in this application?