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Microbiology

Bacteria, viruses, immunology, genetics

100 Q 1 Topics Take Test
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Difficulty: All Easy Medium Hard 51–60 of 100
Topics in Microbiology
All Bacteria & Viruses 100
Q.51 Medium Bacteria & Viruses
A bacterium isolated from a urinary tract infection produces urease enzyme, enabling it to raise urine pH and form struvite crystals. Which organism is most likely?
A Escherichia coli
B Proteus mirabilis
C Klebsiella oxytoca
D Enterococcus faecalis
Correct Answer:  B. Proteus mirabilis
EXPLANATION

Proteus mirabilis produces urease, alkalinizing urine and promoting precipitation of magnesium ammonium phosphate (struvite), forming characteristic staghorn calculi.

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Which viral component is essential for attachment to host cell receptors and is the primary target for neutralizing antibodies?
A Viral envelope lipid bilayer
B Matrix proteins
C Attachment/spike glycoproteins
D Viral DNA/RNA polymerase
Correct Answer:  C. Attachment/spike glycoproteins
EXPLANATION

Attachment proteins (like hemagglutinin in influenza, spike protein in SARS-CoV-2) mediate receptor binding and are immunodominant targets for neutralizing antibodies.

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Q.53 Medium Bacteria & Viruses
A Gram-negative bacterium shows resistance to multiple antibiotics including cephalosporins. The presence of which enzymatic mechanism is most likely responsible?
A Aminoglycoside phosphorylase
B Extended-spectrum beta-lactamase (ESBL)
C Chloramphenicol acetyltransferase
D Erythromycin methylase
Correct Answer:  B. Extended-spectrum beta-lactamase (ESBL)
EXPLANATION

ESBL-producing Gram-negative bacteria (E. coli, Klebsiella) hydrolyze third-generation cephalosporins, conferring resistance to multiple beta-lactam antibiotics.

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Q.54 Medium Bacteria & Viruses
In a viral outbreak investigation, electron microscopy reveals enveloped, pleomorphic particles (80-120 nm) with crown-like spike projections. Which family is most consistent?
A Filoviridae
B Arenaviridae
C Coronaviridae
D Orthomyxoviridae
Correct Answer:  C. Coronaviridae
EXPLANATION

Coronaviruses are characterized by crown-like spike proteins (S protein) on their enveloped surface, which are distinctive under electron microscopy.

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Which bacterial spore-forming genus is most commonly associated with food poisoning and produces heat-resistant endospores?
A Clostridium botulinum
B Bacillus cereus
C Listeria monocytogenes
D Salmonella typhi
Correct Answer:  B. Bacillus cereus
EXPLANATION

Bacillus cereus produces heat-resistant spores surviving cooking temperatures, causing emetic and diarrheal foodborne illness outbreaks.

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Q.56 Medium Bacteria & Viruses
A laboratory identifies a virus with single-stranded RNA genome that must first be transcribed to mRNA before protein synthesis. What is this virus classified as?
A Positive-sense RNA virus (ssRNA+)
B Negative-sense RNA virus (ssRNA-)
C Retrovirus
D Double-stranded RNA virus
Correct Answer:  B. Negative-sense RNA virus (ssRNA-)
EXPLANATION

Negative-sense RNA viruses (-ssRNA) cannot be directly translated; they require RNA-dependent RNA polymerase to synthesize complementary positive-sense mRNA first.

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Q.57 Medium Bacteria & Viruses
In viral pathogenesis, which mechanism allows RNA viruses like influenza to evade host immune response?
A Integration into host chromosome
B Antigenic drift and antigenic shift
C Forming inclusion bodies
D Budding from nuclear membrane
Correct Answer:  B. Antigenic drift and antigenic shift
EXPLANATION

Antigenic drift (point mutations) and antigenic shift (reassortment of gene segments) allow influenza to escape previously acquired immunity, making vaccination necessary annually.

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Which bacterial flagellar arrangement allows movement in a single direction with maximum efficiency?
A Lophotrichous
B Monotrichous
C Peritrichous
D Amphitrichous
Correct Answer:  B. Monotrichous
EXPLANATION

Monotrichous bacteria have a single flagellum at one pole, enabling direct, efficient movement. Peritrichous flagella around the cell body create tumbling motion.

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Q.59 Medium Bacteria & Viruses
A patient presents with community-acquired pneumonia. Culture on MacConkey agar shows non-fermentative, oxidase-positive colonies. Which organism is most likely?
A Streptococcus pneumoniae
B Pseudomonas aeruginosa
C Klebsiella pneumoniae
D Staphylococcus aureus
Correct Answer:  B. Pseudomonas aeruginosa
EXPLANATION

Pseudomonas aeruginosa is a non-fermentative, oxidase-positive, Gram-negative rod commonly associated with respiratory infections and shows characteristic green pigmentation.

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Which of the following bacterial cell wall components is responsible for the Gram-positive purple coloration in Gram staining?
A Thick peptidoglycan layer with teichoic acids
B Thin peptidoglycan with lipopolysaccharide outer membrane
C Mycolic acids and arabinogalactan
D Protein A and surface antigens
Correct Answer:  A. Thick peptidoglycan layer with teichoic acids
EXPLANATION

Gram-positive bacteria have a thick peptidoglycan layer (20-80 nm) with teichoic acids that retains the crystal violet-iodine complex, resulting in purple coloration.

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