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Microbiology

Bacteria, viruses, immunology, genetics

100 Q 1 Topics Take Test
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Difficulty: All Easy Medium Hard 71–80 of 100
Topics in Microbiology
All Bacteria & Viruses 100
A gram-positive coccus forms grape-like clusters and produces alpha-toxin. Which pathogen is being described?
A Streptococcus pneumoniae
B Staphylococcus aureus
C Streptococcus pyogenes
D Enterococcus faecalis
Correct Answer:  B. Staphylococcus aureus
EXPLANATION

Staphylococcus aureus characteristically forms clusters (not chains), produces alpha-hemolysin (alpha-toxin), and is coagulase-positive, distinguishing it from streptococci.

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Q.72 Medium Bacteria & Viruses
Which bacterial flagellar protein is the primary target of monoclonal antibodies used in serological identification?
A Flagellin (H antigen)
B Basal body protein
C Motor proteins
D Hook proteins
Correct Answer:  A. Flagellin (H antigen)
EXPLANATION

Flagellin comprises the helical filament and constitutes the H (Hauch) antigen, used for serotyping bacteria like Salmonella. Other flagellar components are internal and less immunogenic.

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A viral infection shows symptoms of hemorrhagic fever with high mortality. Laboratory tests reveal negative-sense RNA and a helical capsid. Which family of viruses is most likely?
A Flaviviridae
B Filoviridae
C Togaviridae
D Coronaviridae
Correct Answer:  B. Filoviridae
EXPLANATION

Filoviruses (Ebola, Marburg) cause hemorrhagic fevers, possess negative-sense RNA, and display characteristic helical/filamentous morphology with extreme pathogenicity.

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Which virus possesses a segmented genome and is responsible for causing influenza pandemics?
A Influenza A virus
B Rotavirus
C Bunyavirus
D Arenavirus
Correct Answer:  A. Influenza A virus
EXPLANATION

Influenza A virus has 8 RNA segments, enabling antigenic reassortment. This segmentation allows rapid evolution and pandemic potential through genetic exchange with other influenza strains.

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Q.75 Medium Bacteria & Viruses
In a clinical sample, Mycobacterium tuberculosis appears as acid-fast positive. This property is due to which cell wall component?
A Peptidoglycan
B Mycolic acids
C Lipopolysaccharide
D Teichoic acids
Correct Answer:  B. Mycolic acids
EXPLANATION

Mycolic acids in the mycobacterial cell wall resist acid-alcohol decolorization, causing the characteristic acid-fast staining. These long-chain fatty acids are unique to mycobacteria.

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Q.76 Medium Bacteria & Viruses
A researcher observes that a bacterial strain previously sensitive to penicillin now survives treatment. Analysis shows increased production of beta-lactamase. This exemplifies which mechanism of antibiotic resistance?
A Target site modification
B Enzymatic inactivation
C Efflux pump mechanism
D Reduced permeability
Correct Answer:  B. Enzymatic inactivation
EXPLANATION

Beta-lactamase enzymatically cleaves the beta-lactam ring of penicillin, inactivating the antibiotic. This is enzymatic degradation, not target modification or pump-mediated resistance.

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Which bacterial genus produces endospores as a survival mechanism under nutrient-depleted conditions?
A Bacillus and Clostridium
B Staphylococcus and Streptococcus
C Vibrio and Campylobacter
D Salmonella and Shigella
Correct Answer:  A. Bacillus and Clostridium
EXPLANATION

Bacillus and Clostridium are the primary gram-positive bacteria capable of forming endospores. These structures are highly resistant to heat, chemicals, and radiation.

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Q.78 Medium Bacteria & Viruses
A laboratory identifies a virus with positive-sense, single-stranded RNA genome. Which process can this virus directly initiate without viral RNA-dependent RNA polymerase?
A Translation
B Replication
C Reverse transcription
D DNA synthesis
Correct Answer:  A. Translation
EXPLANATION

Positive-sense RNA can directly serve as mRNA for ribosomal translation. Replication requires RNA-dependent RNA polymerase. This genome type doesn't undergo reverse transcription or DNA synthesis.

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Which of the following bacterial structures is responsible for horizontal gene transfer through direct cell-to-cell contact?
A Pili
B Flagella
C Capsule
D Teichoic acid
Correct Answer:  A. Pili
EXPLANATION

Pili (sex pili) mediate bacterial conjugation, enabling direct DNA transfer between cells. Flagella aid motility, capsules provide protection, and teichoic acids are cell wall components.

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Q.80 Medium Bacteria & Viruses
A patient presents with severe respiratory infection. Gram-staining reveals gram-negative rods that are oxidase-positive and motile. Which organism is most likely responsible?
A Pseudomonas aeruginosa
B Escherichia coli
C Klebsiella pneumoniae
D Proteus mirabilis
Correct Answer:  A. Pseudomonas aeruginosa
EXPLANATION

Pseudomonas aeruginosa is a gram-negative, oxidase-positive rod commonly causing respiratory infections in hospitalized patients. E. coli and Klebsiella are oxidase-negative, while Proteus is also oxidase-negative.

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