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NEET UG
NEET Zoology

NEET UG MCQ questions — Physics, Chemistry, Botany & Zoology for medical entrance.

273 Q 4 Subjects 12th (PCB)
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Difficulty: All Easy Medium Hard 1–10 of 273
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Q.1 Medium NEET Zoology Biotechnology & Applications
What is the primary application of AFLP (Amplified Fragment Length Polymorphism) in forensic biotechnology?
A To amplify only coding regions of DNA
B To generate DNA fingerprints for individual identification in criminal investigations
C To sequence entire genomes
D To diagnose genetic diseases only
Correct Answer:  B. To generate DNA fingerprints for individual identification in criminal investigations
EXPLANATION

AFLP generates unique DNA fingerprints by analyzing polymorphic bands through selective amplification and is widely used in forensic identification and paternity testing.

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Q.2 Medium NEET Zoology Biotechnology & Applications
In vaccine development using biotechnology, what is the advantage of mRNA vaccines (like those for COVID-19) over traditional inactivated vaccines?
A They produce stronger side effects
B Faster development, production at scale, and strong immune response without infectious agent
C They can only be administered orally
D They require lifelong booster shots
Correct Answer:  B. Faster development, production at scale, and strong immune response without infectious agent
EXPLANATION

mRNA vaccines can be designed and produced rapidly, manufactured at scale, and trigger robust immune responses without requiring actual pathogenic agents.

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Q.3 Medium NEET Zoology Biotechnology & Applications
What is the significance of the Human Genome Project in biotechnology and personalized medicine?
A It mapped all human genes, enabling identification of disease-causing mutations and development of targeted therapies
B It only sequenced mitochondrial DNA
C It proved that all humans have identical genomes
D It was primarily used for agricultural improvement
Correct Answer:  A. It mapped all human genes, enabling identification of disease-causing mutations and development of targeted therapies
EXPLANATION

The Human Genome Project provided the complete human genetic blueprint, revolutionizing our understanding of genetic diseases and enabling personalized medicine approaches.

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Q.4 Medium NEET Zoology Biotechnology & Applications
Which modern sequencing technology allows for real-time monitoring of DNA synthesis, reducing sequencing time and cost?
A Sanger sequencing
B Next-Generation Sequencing (NGS)
C Nanopore sequencing
D Restriction Fragment Length Polymorphism (RFLP)
Correct Answer:  C. Nanopore sequencing
EXPLANATION

Nanopore sequencing allows real-time DNA reading as strands pass through nanopores, enabling rapid and cost-effective long-read sequencing without amplification.

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Q.5 Medium NEET Zoology Biotechnology & Applications
What is the primary concern with the application of synthetic biology in creating new organisms?
A It is too expensive to implement
B Biosafety and biosecurity risks, including potential creation of harmful organisms
C It requires too much laboratory space
D The public universally supports all synthetic organisms
Correct Answer:  B. Biosafety and biosecurity risks, including potential creation of harmful organisms
EXPLANATION

Synthetic biology raises significant biosafety and biosecurity concerns regarding uncontrolled release of synthetic organisms, potential ecological disruption, and dual-use implications.

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Q.6 Medium NEET Zoology Biotechnology & Applications
What is the principle behind the polymerase chain reaction (PCR) that allows exponential amplification of specific DNA sequences?
A Temperature cycles that denature, allow primer annealing, and enable DNA extension
B Addition of restriction enzymes at each cycle
C Using only one type of nucleotide at a time
D Constant temperature maintenance throughout the process
Correct Answer:  A. Temperature cycles that denature, allow primer annealing, and enable DNA extension
EXPLANATION

PCR works through repeated cycles of three temperatures: denaturation (95°C), annealing (50-65°C), and extension (72°C), with Taq polymerase extending primers exponentially.

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Q.7 Medium NEET Zoology Biotechnology & Applications
Which of the following applications of biotechnology can help in detecting antibiotic-resistant bacteria in clinical samples?
A DNA microarray analysis
B Bioinformatics only
C Gel electrophoresis only
D Manual culture methods only
Correct Answer:  A. DNA microarray analysis
EXPLANATION

DNA microarrays can detect antibiotic resistance genes rapidly by analyzing the genetic profile of bacteria, enabling faster identification compared to traditional culture methods.

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Q.8 Medium NEET Zoology Biotechnology & Applications
In monoclonal antibody production using hybridoma technology, what is the advantage of fusing B cells with myeloma cells?
A The resulting cells can produce a single type of antibody and can divide indefinitely
B The process increases the number of B cells available
C It reduces the cost of antibody production
D The myeloma cells prevent contamination
Correct Answer:  A. The resulting cells can produce a single type of antibody and can divide indefinitely
EXPLANATION

Hybridomas combine the antibody-producing capability of B cells with the immortality of myeloma cancer cells, producing monoclonal antibodies of high specificity indefinitely.

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Q.9 Medium NEET Zoology Biotechnology & Applications
In the context of gene therapy for hereditary diseases, what is the significance of using an inert viral vector that has had its pathogenic genes removed?
A To increase the mutation rate in target cells
B To deliver therapeutic genes while minimizing immune response and pathogenicity
C To prevent the therapeutic gene from being expressed
D To make the viral vector replicate faster
Correct Answer:  B. To deliver therapeutic genes while minimizing immune response and pathogenicity
EXPLANATION

Modified viral vectors with pathogenic genes removed can efficiently deliver therapeutic genes to target cells while being non-pathogenic and reducing immune rejection.

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Q.10 Medium NEET Zoology Biotechnology & Applications
What is the main advantage of using embryonic stem cells (ESCs) in regenerative medicine?
A They are easier to culture than other cell types
B They have unlimited self-renewal capacity and can differentiate into any cell type
C They are found abundantly in adult tissues
D They do not require growth factors for survival
Correct Answer:  B. They have unlimited self-renewal capacity and can differentiate into any cell type
EXPLANATION

Embryonic stem cells (ESCs) are pluripotent, meaning they can self-renew indefinitely and differentiate into any type of cell in the body, making them valuable for regenerative medicine.

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