Home Subjects Biochemistry

Biochemistry

Metabolic pathways, enzymes, proteins

133 Q 3 Topics Take Test
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Difficulty: All Easy Medium Hard 41–50 of 133
Topics in Biochemistry
All Proteins & Enzymes 100 Carbohydrates 100 Lipids 78
Q.41 Medium Carbohydrates
In the pentose phosphate pathway, the oxidative phase generates NADPH. Which metabolic process primarily utilizes this NADPH in fed state?
A Glycolysis
B Ketone body synthesis
C Fatty acid and cholesterol synthesis
D Oxidative phosphorylation
Correct Answer:  C. Fatty acid and cholesterol synthesis
EXPLANATION

NADPH from the oxidative pentose phosphate pathway is essential for reductive biosynthesis of fatty acids and cholesterol, which occur predominantly in the fed state.

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Q.42 Medium Carbohydrates
In hereditary fructose intolerance (HFI), which enzyme deficiency causes accumulation of fructose-1-phosphate?
A Fructokinase
B Aldolase B
C Fructose-1,6-bisphosphatase
D Fructose-1-phosphatase
Correct Answer:  B. Aldolase B
EXPLANATION

HFI results from aldolase B deficiency, preventing cleavage of fructose-1-phosphate into DHAP and glyceraldehyde, leading to accumulation and hepatotoxicity.

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Q.43 Medium Carbohydrates
A newborn presents with hepatomegaly, lactic acidosis, and hypoglycemia. Enzyme analysis shows deficiency of glucose-6-phosphatase. Which disease is this?
A McArdle disease
B Pompe disease
C Von Gierke disease
D Cori disease
Correct Answer:  C. Von Gierke disease
EXPLANATION

Von Gierke disease (GSD Type I) results from glucose-6-phosphatase deficiency, preventing final step of both gluconeogenesis and glycogenolysis, causing severe hypoglycemia.

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Q.44 Medium Carbohydrates
Which of the following is NOT a function of glycogen in liver?
A Maintaining blood glucose levels between meals
B Providing structural support to hepatocytes
C Acting as glucose buffer during fasting
D Supplying glucose-6-phosphate to extrahepatic tissues
Correct Answer:  D. Supplying glucose-6-phosphate to extrahepatic tissues
EXPLANATION

Liver glycogen maintains blood glucose but cannot directly supply glucose-6-phosphate to other tissues as glucose-6-phosphate cannot cross cell membranes.

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Q.45 Medium Carbohydrates
During high-intensity exercise, which carbohydrate provides immediate energy despite low concentration in blood?
A Blood glucose from liver glycogenolysis
B Muscle glycogen
C Circulating fructose
D Dietary disaccharides
Correct Answer:  B. Muscle glycogen
EXPLANATION

Muscle glycogen is the primary energy source during high-intensity exercise because muscle lacks glucose-6-phosphatase and retains glucose-6-phosphate for glycolysis.

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Q.46 Medium Carbohydrates
The branching enzyme in glycogen synthesis catalyzes which type of reaction?
A Removal of α-1,4-glucose units
B Transfer of α-1,4-glucose chain segment to α-1,6 position
C Addition of glucose to growing chain via α-1,4 bond
D Phosphorylation of glucose-6-phosphate
Correct Answer:  B. Transfer of α-1,4-glucose chain segment to α-1,6 position
EXPLANATION

Branching enzyme (amylo-1,6-transglucosidase) transfers segments of α-1,4-linked glucose chains to create α-1,6 branch points in glycogen.

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Q.47 Medium Carbohydrates
A patient presents with inability to metabolize galactose. Which enzyme deficiency is most likely responsible for classical galactosemia?
A Galactokinase deficiency
B Galactose-1-phosphate uridyltransferase deficiency
C UDP-galactose epimerase deficiency
D Lactase deficiency
Correct Answer:  B. Galactose-1-phosphate uridyltransferase deficiency
EXPLANATION

Classical galactosemia results from galactose-1-phosphate uridyltransferase (GALT) deficiency, causing accumulation of galactose-1-phosphate and galactosylated proteins.

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Q.48 Medium Carbohydrates
Which of the following substrates can be directly utilized for glycogen synthesis in the liver?
A Free glucose
B Glucose-1-phosphate
C Glucose-6-phosphate
D Pyruvate
Correct Answer:  B. Glucose-1-phosphate
EXPLANATION

Glucose-1-phosphate is converted to UDP-glucose by UDP-glucose pyrophosphorylase, which is the activated form used by glycogen synthase for glycogen synthesis.

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Q.49 Medium Carbohydrates
In a patient with von Gierke disease, excessive hepatic glucose production is primarily due to:
A Overactive glycogenolysis
B Enhanced glycolysis
C Increased gluconeogenesis compensating for glucose-6-phosphatase deficiency
D Decreased hexokinase activity
Correct Answer:  C. Increased gluconeogenesis compensating for glucose-6-phosphatase deficiency
EXPLANATION

Von Gierke disease (GSD Type I) involves glucose-6-phosphatase deficiency, leading to impaired glucose release. However, alternative glucose production through gluconeogenesis and pentose phosphate pathway increases.

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Q.50 Medium Carbohydrates
Which of the following best explains why glycogen is more soluble than starch despite similar glycosidic linkages?
A Glycogen has more α-1,6 branch points
B Glycogen has shorter outer chains
C Glycogen has β-1,4 linkages
D Glycogen is synthesized in aqueous environments
Correct Answer:  A. Glycogen has more α-1,6 branch points
EXPLANATION

Glycogen has more frequent α-1,6 branch points (approximately every 8-12 glucose units) compared to starch, increasing its solubility and accessibility for enzyme action.

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