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Biochemistry

Metabolic pathways, enzymes, proteins

133 Q 3 Topics Take Test
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Difficulty: All Easy Medium Hard 71–80 of 133
Topics in Biochemistry
All Proteins & Enzymes 100 Carbohydrates 100 Lipids 78
Q.71 Medium Carbohydrates
In the glucose transporter classification, which GLUT protein is responsible for INSULIN-DEPENDENT glucose uptake in muscle and adipose tissue?
A GLUT1
B GLUT2
C GLUT3
D GLUT4
Correct Answer:  D. GLUT4
EXPLANATION

GLUT4 is the insulin-responsive glucose transporter present in skeletal muscle and adipose tissue. Insulin signaling causes GLUT4 translocation to the cell membrane, increasing glucose uptake.

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Q.72 Medium Carbohydrates
Which carbohydrate metabolism enzyme is allosterically inhibited by citrate and ATP but activated by AMP and ADP?
A Pyruvate kinase
B Phosphofructokinase-1
C Hexokinase
D Glyceraldehyde-3-phosphate dehydrogenase
Correct Answer:  B. Phosphofructokinase-1
EXPLANATION

Phosphofructokinase-1 (PFK-1) is the rate-limiting enzyme of glycolysis. It is inhibited by ATP and citrate (signals of sufficient energy) and activated by AMP and ADP (signals of energy depletion).

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Q.73 Medium Carbohydrates
Which enzyme deficiency causes Essential Fructosuria, and why is it clinically benign?
A Aldolase B deficiency; fructose cannot be metabolized
B Fructokinase deficiency; fructose is excreted unchanged in urine without tissue damage
C Hexokinase deficiency; alternative pathways compensate
D Glucokinase deficiency; glucose metabolism is unaffected
Correct Answer:  B. Fructokinase deficiency; fructose is excreted unchanged in urine without tissue damage
EXPLANATION

Essential fructosuria results from fructokinase deficiency. It is benign because fructose is simply excreted in urine without accumulating in tissues or causing metabolic harm.

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Q.74 Medium Carbohydrates
In the pentose phosphate pathway, which intermediate can directly feed into glycolysis as glyceraldehyde-3-phosphate?
A Ribulose-5-phosphate
B Erythrose-4-phosphate
C Fructose-6-phosphate
D Sedoheptulose-7-phosphate
Correct Answer:  C. Fructose-6-phosphate
EXPLANATION

Through the non-oxidative phase of the pentose phosphate pathway, sugars are rearranged to produce fructose-6-phosphate and glyceraldehyde-3-phosphate, which directly enter glycolysis.

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Q.75 Medium Carbohydrates
Which monosaccharide is the primary carbon source for synthesis of heparin and other glycosaminoglycans?
A Glucose
B Galactose
C Glucuronic acid
D Mannose
Correct Answer:  C. Glucuronic acid
EXPLANATION

Glucuronic acid, derived from glucose, is a key component of glycosaminoglycans like heparin, hyaluronic acid, and chondroitin sulfate, providing their acidic properties.

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Q.76 Medium Carbohydrates
A patient presents with lactic acidosis after consuming alcohol. Which enzyme's inhibition by acetaldehyde is primarily responsible for this condition?
A Lactate dehydrogenase
B NAD+-dependent dehydrogenases
C Alcohol dehydrogenase
D Pyruvate carboxylase
Correct Answer:  B. NAD+-dependent dehydrogenases
EXPLANATION

Ethanol metabolism consumes NAD+, reducing the NAD+/NADH ratio. This inhibits NAD+-dependent dehydrogenases including those in gluconeogenesis and lactate oxidation, leading to lactate accumulation.

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Q.77 Medium Carbohydrates
Which of the following statements is TRUE about the Cori cycle?
A It involves the conversion of pyruvate to glucose in muscles
B Lactate produced in muscles is converted to glucose in the liver during rest
C It requires only glycolytic enzymes without gluconeogenic involvement
D It is inactive during intense physical exercise
Correct Answer:  B. Lactate produced in muscles is converted to glucose in the liver during rest
EXPLANATION

The Cori cycle describes lactate produced in muscles during anaerobic glycolysis being transported to the liver where it is converted back to glucose via gluconeogenesis, which is then returned to muscles.

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Q.78 Medium Carbohydrates
In diabetes mellitus type 2, impaired fasting glucose (100-125 mg/dL) is associated with:
A Increased insulin sensitivity
B Insulin resistance and β-cell dysfunction
C Autoimmune destruction of β-cells
D Complete absence of insulin secretion
Correct Answer:  B. Insulin resistance and β-cell dysfunction
EXPLANATION

Type 2 diabetes is characterized by insulin resistance (defective glucose uptake and utilization) and progressive β-cell dysfunction. Impaired fasting glucose (100-125 mg/dL) is a prediabetic state. Fasting glucose ≥126 mg/dL on 2 occasions is diagnostic for diabetes.

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Q.79 Medium Carbohydrates
In the pentose phosphate pathway, the oxidative phase primarily generates:
A ATP and GTP
B NADPH for reductive biosynthesis
C Pyruvate for TCA cycle
D Lactate for gluconeogenesis
Correct Answer:  B. NADPH for reductive biosynthesis
EXPLANATION

The oxidative phase of PPP generates 2 NADPH per glucose-6-phosphate, essential for fatty acid synthesis, cholesterol synthesis, and antioxidant defense (glutathione reduction). This is particularly important in adipose tissue, liver, and RBCs.

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Q.80 Medium Carbohydrates
Cellulose differs from starch in that it contains:
A α(1→4) glycosidic bonds instead of β(1→4)
B β(1→4) glycosidic bonds instead of α(1→4)
C More branching through α(1→6) bonds
D Fructose instead of glucose units
Correct Answer:  B. β(1→4) glycosidic bonds instead of α(1→4)
EXPLANATION

Cellulose contains β(1→4) glycosidic bonds linking glucose units, making it indigestible to humans (no cellulase enzyme). Starch contains α(1→4) bonds, which are digestible.

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