Home Subjects Biochemistry

Biochemistry

Metabolic pathways, enzymes, proteins

278 Q 3 Topics Take Test
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Difficulty: All Easy Medium Hard 101–110 of 278
Topics in Biochemistry
All Proteins & Enzymes 100 Carbohydrates 100 Lipids 78
Q.101 Hard Carbohydrates
In maple syrup urine disease (MSUD), accumulation of branched-chain amino acids affects carbohydrate metabolism by:
A Inhibiting phosphofructokinase
B Competing with glucose uptake transporters
C Inhibiting pyruvate dehydrogenase through altered acetyl-CoA/CoA ratio
D Enhancing glycogen synthase activity
Correct Answer:  C. Inhibiting pyruvate dehydrogenase through altered acetyl-CoA/CoA ratio
EXPLANATION

BCAA metabolism produces acetyl-CoA and increases the acetyl-CoA/CoA ratio, which inhibits pyruvate dehydrogenase, reducing glucose oxidation and affecting carbohydrate metabolism.

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Q.102 Hard Carbohydrates
Which of the following correctly describes the allosteric regulation of glycogen phosphorylase in muscle?
A Active form: phosphorylated; inhibited by AMP
B Active form: dephosphorylated; activated by AMP
C Active form: phosphorylated; activated by AMP
D Active form: unaffected by phosphorylation
Correct Answer:  C. Active form: phosphorylated; activated by AMP
EXPLANATION

In muscle, phosphorylase a (phosphorylated form) is active. It is further activated by AMP, which signals energy depletion during exercise.

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Q.103 Hard Carbohydrates
A patient with G6PD deficiency may develop hemolytic anemia upon exposure to oxidative stress. This occurs because:
A Decreased NADPH reduces glutathione reductase activity
B Decreased ATP impairs active transport
C Increased glycolysis depletes 2,3-DPG
D Impaired lactate production
Correct Answer:  A. Decreased NADPH reduces glutathione reductase activity
EXPLANATION

G6PD deficiency reduces NADPH production, decreasing reduced glutathione (GSH) levels. GSH protects RBC membranes from oxidative damage, so its depletion leads to hemolysis.

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Q.104 Hard Carbohydrates
Which adaptation occurs in skeletal muscle during prolonged fasting (>48 hours)?
A Increased glycogenolysis
B Increased amino acid oxidation and ketone body utilization
C Enhanced glycolysis
D Increased fatty acid synthesis
Correct Answer:  B. Increased amino acid oxidation and ketone body utilization
EXPLANATION

After 24-48 hours of fasting, muscle glycogen is depleted. Muscle shifts to oxidizing amino acids (from proteolysis) and utilizing ketone bodies produced by the liver.

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Q.105 Hard Carbohydrates
In the Cori cycle, lactate produced in muscle is converted to glucose in the liver. Which enzyme is crucial for the final step in hepatic gluconeogenesis?
A Pyruvate kinase
B Glucose-6-phosphatase
C Phosphofructokinase
D Aldolase
Correct Answer:  B. Glucose-6-phosphatase
EXPLANATION

Glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphate to free glucose, which is the final and rate-limiting step of hepatic gluconeogenesis.

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Q.106 Hard Carbohydrates
A 45-year-old male presents with chronic hyperuricemia and gout. Testing reveals elevated lactic acid and hepatomegaly. Which GSD is most likely?
A Type III (Cori disease)
B Type I (Von Gierke disease)
C Type V (McArdle disease)
D Type VII (Tarui disease)
Correct Answer:  B. Type I (Von Gierke disease)
EXPLANATION

Von Gierke disease causes hepatomegaly, lactic acidosis, and hyperuricemia due to glucose-6-phosphatase deficiency, leading to increased glycolysis and purine metabolism.

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Q.107 Medium Carbohydrates
Which of the following substrates can be directly utilized for glycogen synthesis in the liver?
A Free glucose
B Glucose-1-phosphate
C Glucose-6-phosphate
D Pyruvate
Correct Answer:  B. Glucose-1-phosphate
EXPLANATION

Glucose-1-phosphate is converted to UDP-glucose by UDP-glucose pyrophosphorylase, which is the activated form used by glycogen synthase for glycogen synthesis.

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Q.108 Medium Carbohydrates
In a patient with von Gierke disease, excessive hepatic glucose production is primarily due to:
A Overactive glycogenolysis
B Enhanced glycolysis
C Increased gluconeogenesis compensating for glucose-6-phosphatase deficiency
D Decreased hexokinase activity
Correct Answer:  C. Increased gluconeogenesis compensating for glucose-6-phosphatase deficiency
EXPLANATION

Von Gierke disease (GSD Type I) involves glucose-6-phosphatase deficiency, leading to impaired glucose release. However, alternative glucose production through gluconeogenesis and pentose phosphate pathway increases.

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Q.109 Medium Carbohydrates
Which of the following best explains why glycogen is more soluble than starch despite similar glycosidic linkages?
A Glycogen has more α-1,6 branch points
B Glycogen has shorter outer chains
C Glycogen has β-1,4 linkages
D Glycogen is synthesized in aqueous environments
Correct Answer:  A. Glycogen has more α-1,6 branch points
EXPLANATION

Glycogen has more frequent α-1,6 branch points (approximately every 8-12 glucose units) compared to starch, increasing its solubility and accessibility for enzyme action.

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Q.110 Medium Carbohydrates
During the pentose phosphate pathway, which coenzyme is reduced to generate NADPH?
A FAD
B NAD+
C NADP+
D Coenzyme A
Correct Answer:  C. NADP+
EXPLANATION

NADP+ is reduced to NADPH in the oxidative phase of the pentose phosphate pathway, primarily in the G6PD and 6-PGD reactions.

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