Central Exam — General Science
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Q.11 Medium Botany
The phenomenon of leaf fall in deciduous plants before winter is regulated by which hormone?
A Abscisic acid
B Ethylene
C Gibberellin
D Cytokinin
Correct Answer:  A. Abscisic acid
Explanation:

Abscisic acid (ABA) accumulates during water stress and environmental changes, promoting abscission layer formation and leaf fall.

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Q.12 Medium Botany
In plant tissue culture, which growth regulator ratio promotes callus formation?
A High auxin : Low cytokinin
B Low auxin : High cytokinin
C Equal ratio of auxin and cytokinin
D Neither auxin nor cytokinin
Correct Answer:  A. High auxin : Low cytokinin
Explanation:

High auxin to low cytokinin ratio promotes callus formation, while the ratios determine the type of organogenesis.

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Q.13 Medium Botany
The osmotic potential of a solution is directly related to:
A Temperature only
B Solute concentration and temperature
C Pressure only
D Volume of solvent only
Correct Answer:  B. Solute concentration and temperature
Explanation:

Osmotic potential (Ψs) = -iCRT, where it depends on solute concentration (C) and absolute temperature (T).

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Q.14 Medium Botany
Which type of phloem tissue is responsible for translocation of sugars in plants?
A Sieve tube elements
B Companion cells only
C Sieve tube members with companion cells
D Phloem fibers
Correct Answer:  A. Sieve tube elements
Explanation:

# Phloem Tissue and Sugar Translocation

Sieve tube elements are specialized living cells in the phloem that form a continuous network for transporting dissolved sugars and organic compounds throughout the plant.

## Step 1: Understanding Phloem Composition

The phloem tissue consists of multiple cell types, each with different functions in nutrient transport and support.

\[\text{Phloem} = \text{Sieve tube elements} + \text{Companion cells} + \text{Phloem fibers} + \text{Phloem parenchyma}\]

## Step 2: Identifying the Transport Cells

Sieve tube elements are elongated, living cells connected end-to-end through sieve plates (perforated cell walls) that allow rapid movement of sugars and other organic solutes through their cytoplasm via mass flow.

- Companion cells: Support sieve tubes metabolically but don't transport sugars themselves

- Phloem fibers: Provide mechanical support only

- Sieve tube elements: Primary transport vessels with thin cytoplasm lining and functioning sieve pores

The correct answer is (A) Sieve tube elements, as they are the only cells with the structural and functional capacity to translocate sugars bidirectionally throughout the plant.

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Q.15 Medium Botany
In the light-dependent reactions, the splitting of water molecules produces oxygen, hydrogen ions, and:
A NADP+
B NADPH
C Electrons
D ATP alone
Correct Answer:  C. Electrons
Explanation:

Water photolysis (2H2O → O2 + 4H+ + 4e-) releases electrons that are transferred through electron transport chains.

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Q.16 Medium Botany
The enzyme RuBisCO catalyzes the fixation of CO2 by combining it with:
A Glyceraldehyde-3-phosphate
B Ribulose-1,5-bisphosphate
C Phosphoenolpyruvate
D Pyruvate
Correct Answer:  B. Ribulose-1,5-bisphosphate
Explanation:

RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes CO2 fixation with RuBP in the Calvin cycle.

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Q.17 Medium Botany
Which of the following plants exhibits CAM (Crassulacean Acid Metabolism) photosynthesis?
A Wheat
B Rice
C Pineapple
D Maize
Correct Answer:  C. Pineapple
Explanation:

CAM plants like pineapple, cactus, and agave fix CO2 at night into organic acids and release during day for photosynthesis.

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Q.18 Medium Botany
The root pressure phenomenon in plants is primarily due to which of the following?
A Transpiration pull alone
B Capillary action in soil
C Active transport of mineral ions into xylem, creating osmotic gradient
D Gravitational force on water column
Correct Answer:  C. Active transport of mineral ions into xylem, creating osmotic gradient
Explanation:

Root pressure is generated by active transport of mineral ions into the xylem sap, creating a concentration gradient that draws water in osmotically. This is distinct from transpiration pull and is a key concept in plant physiology.

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Q.19 Medium Botany
Which of the following pigments is primarily responsible for absorbing light in the red and far-red regions of the spectrum in photosynthesis?
A Chlorophyll a
B Phytochrome
C Carotenoids
D Xanthophyll
Correct Answer:  B. Phytochrome
Explanation:

# Light Absorption Spectra in Photosynthesis

Phytochrome is a specialized photoreceptor protein that uniquely absorbs light in the red (660 nm) and far-red (730 nm) wavelength regions, making it distinct from other photosynthetic pigments.

## Step 1: Understanding Photoreceptor Types

Different pigments in plants absorb different wavelengths of light based on their molecular structure. Photosynthetic pigments like chlorophyll and carotenoids primarily absorb blue and red light for energy conversion, while phytochrome specifically evolved to detect red and far-red light for signaling purposes rather than energy production.

\[\text{Absorption Peak (Red light)} = 660 \text{ nm} \quad \text{and} \quad \text{Absorption Peak (Far-red light)} = 730 \text{ nm}\]

## Step 2: Comparing Other Options

Chlorophyll a absorbs primarily in blue (430 nm) and red (662 nm) regions for photosynthesis; carotenoids and xanthophyll absorb in blue-green regions (400-550 nm) and serve as accessory pigments. Only phytochrome has its primary functional absorption in the far-red region (730 nm), which is critical for seed germination, flowering, and shade avoidance responses in plants.

\[\text{Phytochrome absorption spectrum} = \text{Red (660 nm)} + \text{Far-red (730 nm)} \text{ regions}\]

The correct answer is (B) Phytochrome, as it is the only pigment primarily responsible for absorbing light specifically in both the red and far-red regions of the electromagnetic spectrum.

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Q.20 Medium Botany
In C4 photosynthesis, the first stable compound formed after CO2 fixation is:
A 3-Phosphoglycerate (3-PGA)
B Oxaloacetate
C Ribulose-1,5-bisphosphate
D Glucose
Correct Answer:  B. Oxaloacetate
Explanation:

In C4 plants, PEP carboxylase fixes CO2 into oxaloacetate (4-carbon compound), unlike C3 plants where RuBisCO produces 3-PGA. This adaptation reduces photorespiration and is efficient in hot climates.

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