Glycogen is primarily stored in which of the following tissues in humans?

The correct answer is B: Liver and skeletal muscles. Glycogen, the storage polysaccharide in animals, is primarily stored in the liver (100-120g) and skeletal muscles (400-500g). The liver glycogen maintains blood glucose, while muscle glycogen is used locally.

In the pentose phosphate pathway, the oxidative phase primarily generates:

The correct answer is B: NADPH for reductive biosynthesis. The oxidative phase of PPP generates 2 NADPH per glucose-6-phosphate, essential for fatty acid synthesis, cholesterol synthesis, and antioxidant defense (glutathione reduction). This is particularly important in adipose tissue, liver, and RBCs.

Which post-translational modification is essential for the activation of blood clotting cascade and involves γ-carboxylation of glutamate residues?

The correct answer is C: Hydroxylation and carboxylation (requires vitamin K as cofactor). Vitamin K-dependent carboxylation of glutamate residues in prothrombin and other clotting factors creates γ-carboxyglutamate residues that coordinate Ca2+ ions, essential for binding to phospholipid membranes and clotting cascade progression.

Which of the following is a reducing sugar?

The correct answer is C: Maltose. Maltose is a reducing sugar because it has a free anomeric carbon. Sucrose and trehalose are non-reducing sugars as both anomeric carbons are involved in glycosidic bonds.

Which of the following statements about saturated fatty acids is correct?

The correct answer is A: They have lower melting points than unsaturated fatty acids. Saturated fatty acids have higher melting points and are typically solid at room temperature due to tight packing. They are more stable and less prone to oxidation than unsaturated fatty acids.

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Biochemistry

Metabolic pathways, enzymes, proteins

133 Q 3 Topics Take Mock Test

Difficulty: All Easy Medium Hard 131–133 of 133

Topics in Biochemistry

All Proteins & Enzymes 100 Carbohydrates 100 Lipids 78

Q.131 Medium Proteins & Enzymes

Which type of enzyme inhibition is characterized by a competitive interaction with the active site?

A Non-competitive inhibition

B Uncompetitive inhibition

C Allosteric inhibition

D Competitive inhibition

Correct Answer: D. Competitive inhibition

EXPLANATION

In competitive inhibition, the inhibitor competes with substrate for the active site and can be overcome by increasing substrate concentration.

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Q.132 Medium Proteins & Enzymes

The Michaelis-Menten equation describes enzyme kinetics. What does Km represent?

A Maximum velocity of the enzyme

B Substrate concentration at half maximal velocity

C Enzyme concentration

D Catalytic constant

Correct Answer: B. Substrate concentration at half maximal velocity

EXPLANATION

Km (Michaelis constant) represents the substrate concentration at which enzyme velocity is half of Vmax, indicating substrate affinity.

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Q.133 Medium Proteins & Enzymes

Enzyme cofactors are non-protein substances that are essential for enzyme activity. Which of the following is NOT a cofactor?

A Metal ions like Zn²⁺

B Coenzyme A

C NAD⁺

D Serine

Correct Answer: D. Serine

EXPLANATION

Serine is an amino acid, not a cofactor. Cofactors include metal ions and coenzymes like NAD⁺, FAD, and CoA.

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