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Q.81 Medium Botany
The enzyme RuBisCO catalyzes the fixation of CO2 by combining it with:
A Glyceraldehyde-3-phosphate
B Ribulose-1,5-bisphosphate
C Phosphoenolpyruvate
D Pyruvate
Correct Answer:  B. Ribulose-1,5-bisphosphate
EXPLANATION

RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes CO2 fixation with RuBP in the Calvin cycle.

Test
Q.82 Medium Botany
In the light-dependent reactions, the splitting of water molecules produces oxygen, hydrogen ions, and:
A NADP+
B NADPH
C Electrons
D ATP alone
Correct Answer:  C. Electrons
EXPLANATION

Water photolysis (2H2O → O2 + 4H+ + 4e-) releases electrons that are transferred through electron transport chains.

Test
Q.83 Medium Botany
Which type of phloem tissue is responsible for translocation of sugars in plants?
A Sieve tube elements
B Companion cells only
C Sieve tube members with companion cells
D Phloem fibers
Correct Answer:  A. Sieve tube elements
EXPLANATION

# Phloem Tissue and Sugar Translocation

Sieve tube elements are specialized living cells in the phloem that form a continuous network for transporting dissolved sugars and organic compounds throughout the plant.

## Step 1: Understanding Phloem Composition

The phloem tissue consists of multiple cell types, each with different functions in nutrient transport and support.

\[\text{Phloem} = \text{Sieve tube elements} + \text{Companion cells} + \text{Phloem fibers} + \text{Phloem parenchyma}\]

## Step 2: Identifying the Transport Cells

Sieve tube elements are elongated, living cells connected end-to-end through sieve plates (perforated cell walls) that allow rapid movement of sugars and other organic solutes through their cytoplasm via mass flow.

- Companion cells: Support sieve tubes metabolically but don't transport sugars themselves

- Phloem fibers: Provide mechanical support only

- Sieve tube elements: Primary transport vessels with thin cytoplasm lining and functioning sieve pores

The correct answer is (A) Sieve tube elements, as they are the only cells with the structural and functional capacity to translocate sugars bidirectionally throughout the plant.

Test
Q.84 Medium Botany
The osmotic potential of a solution is directly related to:
A Temperature only
B Solute concentration and temperature
C Pressure only
D Volume of solvent only
Correct Answer:  B. Solute concentration and temperature
EXPLANATION

Osmotic potential (Ψs) = -iCRT, where it depends on solute concentration (C) and absolute temperature (T).

Test
Q.85 Medium Botany
In plant tissue culture, which growth regulator ratio promotes callus formation?
A High auxin : Low cytokinin
B Low auxin : High cytokinin
C Equal ratio of auxin and cytokinin
D Neither auxin nor cytokinin
Correct Answer:  A. High auxin : Low cytokinin
EXPLANATION

High auxin to low cytokinin ratio promotes callus formation, while the ratios determine the type of organogenesis.

Test
Q.86 Medium Botany
The phenomenon of leaf fall in deciduous plants before winter is regulated by which hormone?
A Abscisic acid
B Ethylene
C Gibberellin
D Cytokinin
Correct Answer:  A. Abscisic acid
EXPLANATION

Abscisic acid (ABA) accumulates during water stress and environmental changes, promoting abscission layer formation and leaf fall.

Test
Q.87 Medium Botany
Which of the following statements about stomatal opening is correct?
A Guard cells lose turgor pressure when stomata open
B Potassium ions accumulate in guard cells during stomatal opening
C Stomata open in complete darkness
D Guard cells have thick outer walls and thin inner walls
Correct Answer:  B. Potassium ions accumulate in guard cells during stomatal opening
EXPLANATION

During stomatal opening, potassium ions (K+) actively accumulate in guard cells, increasing osmotic potential and causing water influx.

Test
Q.88 Medium Botany
The root pressure phenomenon in plants can be measured using a device called:
A Potometer
B Barograph
C Manometer
D Tensiometer
Correct Answer:  C. Manometer
EXPLANATION

Root pressure is measured using a manometer connected to the cut end of a plant stem, showing the positive pressure generated by roots.

Test
Q.89 Medium Botany
In C4 plants, the first stable compound formed during carbon fixation is:
A 3-Phosphoglycerate
B Oxaloacetate
C Ribulose-1,5-bisphosphate
D Glucose
Correct Answer:  B. Oxaloacetate
EXPLANATION

In C4 photosynthesis, CO2 combines with PEP (3-carbon) to form oxaloacetate (4-carbon compound) via the enzyme PEP carboxylase.

Test
Q.90 Medium Human Body
Universal blood donor group?
A A+
B B+
C AB+
D O-
Correct Answer:  D. O-
EXPLANATION

O- has no antigens, universal donor.

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