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NEET Botany
Biotechnology in Plants

Botany questions for NEET UG — Plant Anatomy, Physiology, Reproduction, Ecology.

10 Q 7 Topics Take Mock Test
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Topics in NEET Botany
Bt cotton produces Cry1Ac protein that is toxic to lepidopteran insects. Which of the following statements correctly describes the mechanism of toxin action?
A Cry protein binds to alkaline midgut receptors and creates pores in the intestinal epithelium
B Cry protein inhibits acetylcholinesterase enzyme in the insect nervous system
C Cry protein prevents DNA replication in insect cells
D Cry protein blocks ATP synthesis in insect mitochondria
Correct Answer:  A. Cry protein binds to alkaline midgut receptors and creates pores in the intestinal epithelium
EXPLANATION

Cry proteins from Bacillus thuringiensis work by binding to specific receptors in the alkaline midgut of susceptible insects, forming pores that lead to cell lysis and death. This is the well-established mechanism of Bt toxin action, making it selective for certain insect orders.

Test
Which regulatory body in India approves genetically modified crops?
A ICAR
B Genetic Engineering Appraisal Committee (GEAC)
C Ministry of Agriculture
D Indian Standards Bureau
Correct Answer:  B. Genetic Engineering Appraisal Committee (GEAC)
EXPLANATION

The GEAC under the Ministry of Environment, Forest and Climate Change approves GM crops in India after safety and efficacy assessment.

Test
What is a marker-assisted selection (MAS) in plant breeding?
A Visual selection of plants based on phenotype
B Selection of plants using DNA markers linked to desired traits
C Random selection of plants
D Selection based on plant height
Correct Answer:  B. Selection of plants using DNA markers linked to desired traits
EXPLANATION

MAS uses molecular markers (DNA sequences) to identify and select plants carrying desired genes without waiting for phenotypic expression.

Test
What is the significance of the T-DNA in Agrobacterium tumefaciens?
A It codes for bacterial growth
B It is the DNA segment transferred into plant cells
C It synthesizes plant hormones
D It provides antibiotic resistance to bacteria
Correct Answer:  B. It is the DNA segment transferred into plant cells
EXPLANATION

T-DNA (Transfer DNA) is the specific segment of the Ti plasmid that integrates into the plant genome during Agrobacterium-mediated transformation.

Test
Which of the following is a somaclone?
A A plant derived from sexual reproduction
B A plant obtained through tissue culture from a single parent
C A hybrid plant variety
D A polyploid plant
Correct Answer:  B. A plant obtained through tissue culture from a single parent
EXPLANATION

Somaclones are plants regenerated from somatic cells via tissue culture, genetically identical to the parent plant unless mutations occur.

Test
Which hormone ratio favors shoot formation in plant tissue culture?
A High auxin : low cytokinin
B Low auxin : high cytokinin
C Equal ratio of both
D High gibberellin concentration
Correct Answer:  B. Low auxin : high cytokinin
EXPLANATION

A low auxin to high cytokinin ratio promotes shoot formation, while the opposite (high auxin : low cytokinin) promotes root formation.

Test
What is the primary purpose of callus tissue in plant biotechnology?
A Energy production
B Source for regenerating whole plants
C Hormone synthesis
D Nutrient storage
Correct Answer:  B. Source for regenerating whole plants
EXPLANATION

Callus is undifferentiated plant tissue used in micropropagation and plant regeneration through somatic embryogenesis or organogenesis.

Test
Golden Rice is transgenic rice enriched with which nutrient?
A Iron
B Calcium
C Beta-carotene (Vitamin A precursor)
D Vitamin C
Correct Answer:  C. Beta-carotene (Vitamin A precursor)
EXPLANATION

Golden Rice contains genes for beta-carotene production, making it rich in Vitamin A, addressing nutritional deficiency in rice-consuming populations.

Test
Which method of plant genetic engineering does not require a vector?
A Microprojectile bombardment
B Agrobacterium-mediated transformation
C Electroporation
D A and C both
Correct Answer:  D. A and C both
EXPLANATION

Both microprojectile bombardment (gene gun) and electroporation are direct DNA delivery methods that don't require a biological vector.

Test
Which of the following crops is NOT approved for cultivation as Bt transgenic in India?
A Bt Cotton
B Bt Brinjal
C Bt Maize
D Bt Rice
Correct Answer:  D. Bt Rice
EXPLANATION

As of 2024-25, only Bt Cotton is commercially cultivated in India. Bt Brinjal has regulatory issues, and Bt Rice/Maize are not approved yet.

Test
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