Which of the following restriction enzymes produces sticky ends that are compatible with EcoRI sticky ends?

The correct answer is C: MboI. MboI produces sticky ends identical to EcoRI due to recognition of similar DNA sequences (GATC), making them compatible for ligation.

Which hormone is most critical for cell growth and proliferation in serum-containing culture media?

The correct answer is C: Growth factors present in serum (FBS). Fetal Bovine Serum (FBS) contains essential growth factors, hormones, and attachment factors that promote cell growth and proliferation in culture.

In the production of recombinant human insulin using genetically engineered bacteria, which organism is primarily used?

The correct answer is B: Escherichia coli (E. coli). E. coli is the primary organism used for recombinant insulin production due to its well-characterized genetics, rapid growth, and ability to produce high levels of recombinant proteins.

Which DNA fingerprinting technique using PCR amplification of repetitive sequences is widely used in paternity testing and criminal investigations in India?

The correct answer is B: STR (Short Tandem Repeat) analysis. STR profiling amplifies variable number tandem repeats (VNTRs) and is the gold standard for DNA fingerprinting in forensics, recognized by Indian law enforcement and courts.

Home

Home › Subjects › Biotechnology › Cell Culture

Biotechnology
Cell Culture

Genetic engineering, fermentation, cell biology

15 Q 3 Topics Take Mock Test

Difficulty: All Easy Medium Hard 11–15 of 15

Topics in Biotechnology

All Genetic Engineering 100 PCR & DNA Technology 100 Cell Culture 60

Q.11 Hard Cell Culture

What is the significance of mycoplasma testing in cell culture quality control for biopharmaceutical production?

A Mycoplasma detection is optional and rarely performed

B Mycoplasma contamination is easily visible under light microscope

C Mycoplasma is a critical contaminant that requires detection by PCR or ELISA to ensure product safety and efficacy

D Mycoplasma only affects bacterial cultures, not mammalian cells

Correct Answer: C. Mycoplasma is a critical contaminant that requires detection by PCR or ELISA to ensure product safety and efficacy

EXPLANATION

Mycoplasma is an obligatory contaminant in cell cultures that can affect cell behavior and product quality. Detection via PCR, culture, or ELISA is mandatory in biopharmaceutical manufacturing per ICH guidelines.

Test

Q.12 Hard Cell Culture

Which of the following represents the correct sequence in scaling up cell culture from laboratory to industrial bioreactor?

A Flask → 10L bioreactor → 100L bioreactor → 1000L bioreactor

B 1000L bioreactor → 100L bioreactor → 10L bioreactor → Flask

C 10L bioreactor → Flask → 100L bioreactor → 1000L bioreactor

D All scales can be done simultaneously without sequence

Correct Answer: A. Flask → 10L bioreactor → 100L bioreactor → 1000L bioreactor

EXPLANATION

Scale-up follows a logical progression from small (flask) to larger volumes, with each step validated for maintaining cell viability, growth rate, and product quality.

Test

Q.13 Hard Cell Culture

In the context of vaccine production using cell culture, which cell lines are most commonly used in India?

A Only bacterial cultures

B Vero cells, CHO cells, and HEK293 cells

C Fungal cultures exclusively

D Plant callus tissues only

Correct Answer: B. Vero cells, CHO cells, and HEK293 cells

EXPLANATION

Vero (monkey kidney), CHO (hamster ovary), and HEK293 (human embryonic kidney) cells are widely used for vaccine production including COVID-19 and other vaccines in India.

Test

Q.14 Hard Cell Culture

A researcher observes that their CHO (Chinese Hamster Ovary) cell culture shows reduced protein production despite maintaining normal cell density. What could be the most likely cause?

A Excessive CO₂ concentration above 10%

B Suboptimal osmolarity or nutrient depletion in the medium

C Temperature too high (above 40°C)

D Presence of antibiotics in the medium

Correct Answer: B. Suboptimal osmolarity or nutrient depletion in the medium

EXPLANATION

Even at normal density, cells require optimal osmolarity (280-320 mOsm/kg) and adequate nutrients. Nutrient depletion reduces metabolic activity and protein synthesis despite maintained cell count.

Test

Q.15 Hard Cell Culture

What is the primary limitation of primary cell cultures compared to continuous cell lines?

A They are more expensive to establish initially

B They have limited lifespan and gradually lose original characteristics

C They cannot be used for any research purpose

D They grow faster than continuous cell lines

Correct Answer: B. They have limited lifespan and gradually lose original characteristics

EXPLANATION

Primary cells have limited replicative lifespan due to the Hayflick limit and may undergo changes over passages, whereas continuous cell lines can divide indefinitely.

Test

1 2

All Subjects & Topics

Govt. Exams

Entrance Exams

Teacher Exams

Subjects

Quick Links

Biotechnology Cell Culture

Biotechnology
Cell Culture