Which of the following regulatory bodies in India is responsible for the approval of GM crops?

The correct answer is B: Genetic Engineering Appraisal Committee (GEAC) under the Ministry of Environment, Forest and Climate Change. The GEAC (Genetic Engineering Appraisal Committee) is the apex body in India that approves the environmental release and commercialization of GM crops after rigorous evaluation.

What is the primary advantage of using homologous recombination over random integration in genetic engineering?

The correct answer is B: It allows targeted gene insertion at specific genomic locations. Homologous recombination enables precise insertion of genes at specific genomic locations with high accuracy, reducing off-target effects. This is more predictable than random integration.

Which bacterial species is commonly used as a host for expressing recombinant human proteins in industrial biotechnology?

The correct answer is B: Escherichia coli. E. coli is the preferred expression host for many therapeutic proteins including insulin and growth factors due to its rapid growth, well-characterized genetics, and established fermentation technology.

In PCR, the denaturation step typically occurs at:

The correct answer is B: 94-95°C. Denaturation separates double-stranded DNA into single strands, requiring high temperatures of 94-95°C.

Which of the following represents the correct sequence in scaling up cell culture from laboratory to industrial bioreactor?

The correct answer is A: Flask → 10L bioreactor → 100L bioreactor → 1000L bioreactor. Scale-up follows a logical progression from small (flask) to larger volumes, with each step validated for maintaining cell viability, growth rate, and product quality.

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Biotechnology
Cell Culture

Genetic engineering, fermentation, cell biology

15 Q 3 Topics Take Mock Test

Difficulty: All Easy Medium Hard 11–15 of 15

Topics in Biotechnology

All Genetic Engineering 100 PCR & DNA Technology 100 Cell Culture 60

Q.11 Easy Cell Culture

Which growth factor is primarily responsible for promoting cell proliferation in mammalian cell culture media?

A Epidermal Growth Factor (EGF)

B Insulin

C Transferrin

D Albumin

Correct Answer: A. Epidermal Growth Factor (EGF)

EXPLANATION

EGF is a primary mitogenic growth factor that stimulates cell proliferation. While other factors support cell survival, EGF is specifically responsible for promoting cell division in mammalian cultures.

Test

Q.12 Easy Cell Culture

What is the normal pH range required for optimal mammalian cell culture?

A 4.5-5.5

B 7.2-7.4

C 8.5-9.0

D 6.0-6.5

Correct Answer: B. 7.2-7.4

EXPLANATION

Mammalian cells require a pH of 7.2-7.4 (physiological pH) for optimal growth. This is maintained using buffers like bicarbonate or HEPES in culture media.

Test

Q.13 Easy Cell Culture

Which of the following characteristics is essential for continuous cell lines?

A Limited lifespan of 20-30 divisions

B Unlimited replicative potential and ability to divide indefinitely

C Requirement for fresh serum every 6 hours

D Complete inability to survive in artificial media

Correct Answer: B. Unlimited replicative potential and ability to divide indefinitely

EXPLANATION

Continuous cell lines (like HeLa, CHO) have unlimited replicative potential due to telomerase activation or genetic modifications, allowing indefinite division unlike primary cultures.

Test

Q.14 Easy Cell Culture

What is the primary purpose of using a laminar flow hood in cell culture?

A To increase the temperature of the culture medium

B To maintain aseptic conditions and prevent contamination

C To enhance cell growth rate by 50%

D To reduce the cost of culture media

Correct Answer: B. To maintain aseptic conditions and prevent contamination

EXPLANATION

Laminar flow hoods create unidirectional airflow through HEPA filters, maintaining a sterile environment and preventing microbial contamination during cell culture operations.

Test

Q.15 Easy Cell Culture

Which of the following is the most commonly used culture medium for animal cell culture in laboratories?

A DMEM (Dulbecco's Modified Eagle Medium)

B Luria-Bertani broth

C Potato dextrose agar

D Czapek Dox medium

Correct Answer: A. DMEM (Dulbecco's Modified Eagle Medium)

EXPLANATION

DMEM is the most widely used culture medium for mammalian/animal cell culture due to its balanced nutrient composition. LB broth is for bacteria, PDA for fungi, and Czapek Dox for fungi.

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Cell Culture