In insulin production using genetically engineered E. coli, why is the use of proinsulin preferred over direct insulin gene expression?

The correct answer is C: Proinsulin can be properly folded in bacteria; mature insulin requires post-translational processing. E. coli lacks proteases and post-translational modification machinery. Proinsulin can fold properly in bacteria; it's then enzymatically cleaved to produce mature, bioactive insulin.

In DNA barcoding for species identification, which gene region is most commonly used?

The correct answer is B: Cytochrome c oxidase subunit I (COI). COI (cytochrome c oxidase I) is the standard DNA barcode for animal identification due to its high interspecific variation and low intraspecific variation.

In synthetic biology, what is the primary advantage of using BioBricks standardized parts over conventional genetic engineering?

The correct answer is B: Interchangeable, modular design enables rapid prototyping of genetic circuits with predictable behavior. BioBricks provide standardized interfaces (RFC10 standards) for combining biological parts into systems, enabling composable genetic design, rapid iteration, and community resource sharing.

Which of the following is NOT a component of basal culture media?

The correct answer is C: Monoclonal antibodies. Basal media contain amino acids, vitamins, inorganic salts, and carbohydrates. Monoclonal antibodies are added supplements, not basal media components.

Which technique is used to identify whether a gene sequence contains introns and exons in eukaryotic organisms?

The correct answer is B: Heteroduplex analysis. Heteroduplex analysis uses electron microscopy to visualize DNA-RNA hybrids. Introns form loops that are clearly visible, confirming their presence.

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Biotechnology
PCR & DNA Technology

Genetic engineering, fermentation, cell biology

22 Q 3 Topics Take Mock Test

Difficulty: All Easy Medium Hard 21–22 of 22

Topics in Biotechnology

All Genetic Engineering 100 PCR & DNA Technology 100 Cell Culture 60

Q.21 Easy PCR & DNA Technology

In PCR, the denaturation step typically occurs at:

A 50-65°C

B 94-95°C

C 72-75°C

D 30-40°C

Correct Answer: B. 94-95°C

EXPLANATION

Denaturation separates double-stranded DNA into single strands, requiring high temperatures of 94-95°C.

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Q.22 Easy PCR & DNA Technology

Which enzyme is responsible for synthesizing new DNA strands during PCR?

A Taq DNA polymerase

B Restriction endonuclease

C DNA ligase

D Helicase

Correct Answer: A. Taq DNA polymerase

EXPLANATION

Taq DNA polymerase from Thermus aquaticus is heat-stable and essential for DNA synthesis in PCR cycles.

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Biotechnology PCR & DNA Technology

Biotechnology
PCR & DNA Technology