What is the primary advantage of using homologous recombination over random integration in genetic engineering?

The correct answer is B: It allows targeted gene insertion at specific genomic locations. Homologous recombination enables precise insertion of genes at specific genomic locations with high accuracy, reducing off-target effects. This is more predictable than random integration.

Which technique would be most suitable for introducing multiple genes simultaneously into a plant cell?

The correct answer is B: Gene stacking or pyramiding using multiple constructs. Gene stacking or pyramiding involves incorporating multiple transgenes into a single organism, useful for combining multiple desired traits like herbicide resistance and pest resistance.

In gene editing, CRISPR-Cas9 uses which type of guide molecule?

The correct answer is B: Guide RNA (sgRNA). CRISPR-Cas9 employs a single guide RNA (sgRNA) that directs Cas9 endonuclease to specific DNA target sequences for precise editing.

For Indian biotech industries (2024-25) producing therapeutic monoclonal antibodies, which regulatory requirement regarding cell line characterization is mandatory?

The correct answer is B: Complete characterization including karyotype, mycoplasma testing, and adventitious agent testing per ICH guidelines. ICH Q5A/Q5B guidelines (followed by India's DCGI) mandate comprehensive cell line characterization including identity verification, sterility, mycoplasma, and viral testing for therapeutic products.

Home

Home › Subjects › Biotechnology › Genetic Engineering

Biotechnology
Genetic Engineering

Genetic engineering, fermentation, cell biology

26 Q 3 Topics Take Mock Test

Difficulty: All Easy Medium Hard 21–26 of 26

Topics in Biotechnology

All Genetic Engineering 100 PCR & DNA Technology 100 Cell Culture 60

Q.21 Easy Genetic Engineering

Which enzyme is commonly used as a molecular scissors in CRISPR-Cas9 gene editing technology?

A Cas9 endonuclease

B DNA polymerase

C Reverse transcriptase

D Ligase

Correct Answer: A. Cas9 endonuclease

EXPLANATION

Cas9 is an endonuclease enzyme that cuts DNA at specific sites guided by RNA. DNA polymerase synthesizes DNA, reverse transcriptase converts RNA to DNA, and ligase seals DNA strands.

Test

Q.22 Easy Genetic Engineering

Which Indian-approved genetically modified crop is widely cultivated for herbicide resistance?

A Golden Rice

B Bt Brinjal

C Bt Cotton

D Herbicide-resistant Mustard (HT Mustard)

Correct Answer: D. Herbicide-resistant Mustard (HT Mustard)

EXPLANATION

HT Mustard (DHM-11) was approved for cultivation in India in 2022, engineered with herbicide tolerance for better weed management.

Test

Q.23 Easy Genetic Engineering

What is the main advantage of using Bt cotton in India?

A Increased photosynthesis

B Resistance to Helicoverpa armigera (cotton bollworm)

C Enhanced water absorption

D Improved fiber color

Correct Answer: B. Resistance to Helicoverpa armigera (cotton bollworm)

EXPLANATION

Bt cotton contains the Cry1Ac gene from Bacillus thuringiensis, which produces toxins lethal to cotton bollworm larvae, reducing pesticide use significantly.

Test

Q.24 Easy Genetic Engineering

Which of the following is an example of a vector used in genetic engineering?

A Plasmid

B Ribosome

C Mitochondrion

D Lysosome

Correct Answer: A. Plasmid

EXPLANATION

Plasmids are circular DNA molecules found in bacteria that can be used as vectors to carry foreign DNA into host cells during genetic engineering.

Test

Q.25 Easy Genetic Engineering

What is the primary purpose of using DNA ligase in genetic engineering?

A To separate DNA strands

B To join DNA fragments by forming phosphodiester bonds

C To replicate DNA molecules

D To denature DNA

Correct Answer: B. To join DNA fragments by forming phosphodiester bonds

EXPLANATION

DNA ligase catalyzes the formation of phosphodiester bonds between adjacent DNA fragments, sealing nicks in the DNA backbone after recombination.

Test

Q.26 Easy Genetic Engineering

Which enzyme is commonly used to cut DNA at specific recognition sequences during genetic engineering?

A Restriction endonuclease

B DNA polymerase

C Helicase

D Ligase

Correct Answer: A. Restriction endonuclease

EXPLANATION

Restriction endonucleases (restriction enzymes) recognize and cut DNA at specific palindromic sequences, making them essential tools in genetic engineering for creating recombinant DNA.

Test

1 2 3

All Subjects & Topics

Govt. Exams

Entrance Exams

Teacher Exams

Subjects

Quick Links

Biotechnology Genetic Engineering

Biotechnology
Genetic Engineering