In next-generation sequencing-based CRISPR screening, what does 'pooled library screening' allow researchers to identify?

The correct answer is B: Genes involved in specific phenotypes among thousands of edited variants simultaneously. Pooled CRISPR screening uses large libraries of guide RNAs to simultaneously test thousands of genetic perturbations, allowing identification of genes associated with specific phenotypes through NGS readout.

What is the significance of using antifoam agents in large-scale bioreactors?

The correct answer is B: To prevent foam formation which can cause cell damage and overflow. Foam formation in bioreactors during aeration can cause cell lysis, loss of surface area, and carryover into exhaust lines. Antifoam agents (silicone-based) prevent this.

The principle of AFLP (Amplified Fragment Length Polymorphism) combines:

The correct answer is A: Restriction digestion and PCR amplification. AFLP integrates restriction enzyme digestion with selective PCR amplification of restriction fragments for genetic diversity analysis.

In a multiplex PCR assay designed to simultaneously amplify 6 different STR (short tandem repeat) loci for forensic DNA profiling, what is the critical consideration that must be addressed?

The correct answer is A: All primers must have identical Tm values and amplicons of similar sizes to avoid preferential amplification. In multiplex PCR, primers with similar Tm values ensure equal amplification efficiency across all targets. Amplicons of comparable sizes prevent preferential amplification of shorter fragments (which amplify faster), ensuring balanced and accurate STR profiles for individual identification.

Which enzyme is responsible for synthesizing new DNA strands during PCR?

The correct answer is A: Taq DNA polymerase. Taq DNA polymerase from Thermus aquaticus is heat-stable and essential for DNA synthesis in PCR cycles.

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Biotechnology

Genetic engineering, fermentation, cell biology

63 Q 3 Topics Take Mock Test

Difficulty: All Easy Medium Hard 61–63 of 63

Topics in Biotechnology

All Genetic Engineering 100 PCR & DNA Technology 100 Cell Culture 60

Q.61 Easy Genetic Engineering

Which of the following is an example of a vector used in genetic engineering?

A Plasmid

B Ribosome

C Mitochondrion

D Lysosome

Correct Answer: A. Plasmid

EXPLANATION

Plasmids are circular DNA molecules found in bacteria that can be used as vectors to carry foreign DNA into host cells during genetic engineering.

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Q.62 Easy Genetic Engineering

What is the primary purpose of using DNA ligase in genetic engineering?

A To separate DNA strands

B To join DNA fragments by forming phosphodiester bonds

C To replicate DNA molecules

D To denature DNA

Correct Answer: B. To join DNA fragments by forming phosphodiester bonds

EXPLANATION

DNA ligase catalyzes the formation of phosphodiester bonds between adjacent DNA fragments, sealing nicks in the DNA backbone after recombination.

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Q.63 Easy Genetic Engineering

Which enzyme is commonly used to cut DNA at specific recognition sequences during genetic engineering?

A Restriction endonuclease

B DNA polymerase

C Helicase

D Ligase

Correct Answer: A. Restriction endonuclease

EXPLANATION

Restriction endonucleases (restriction enzymes) recognize and cut DNA at specific palindromic sequences, making them essential tools in genetic engineering for creating recombinant DNA.

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